Glycolate Oxidase Activity in Algae
نویسندگان
چکیده
منابع مشابه
Glycolate oxidase activity in algae.
The g1lycolate oxidase reactioni by x-hich gly colate is oxidized to glyoxylate (3, 10) is catailyzed by a flavoprotein (18) th,at is widely distributed in higher plants (12) and fungi (4). Its presence is g,elleralllly assumed in all phoiosynithetic tissuies, since glycolate is an early product of photosynthesis (14), yet this substrate is tusually found in oiily minute amounits. The oxidation...
متن کاملGlycolate pathway in algae.
No glycolate oxidase activity could be detected by manometric, isotopic, or spectrophotometric techniques in cell extracts from 5 strains of algae grown in the light with CO(2). However, NADH:glyoxylate reductase, phosphoglycolate phosphatase and isocitrate dehydrogenase were detected in the cell extracts. The serine formed by Chlorella or Chlamydomonas after 12 seconds of photosynthetic (14)CO...
متن کاملThe evolution of photorespiratory glycolate oxidase activity.
The first step of photosynthetic carbon fixation is catalyzed by ribulose-1,5bisphosphate carboxylase/oxygenase, which arose when the atmosphere contained virtually no O2. The increase of atmospheric O2 caused by oxygenic photosynthesis gave rise to photorespiratory metabolism, a process whereby O2 substitutes for CO2, causing ribulose-1,5bisphosphate carboxylase/oxygenase to produce the toxic ...
متن کاملHigh glycolate oxidase activity is required for survival of maize in normal air.
A mutant in the maize (Zea mays) Glycolate Oxidase1 (GO1) gene was characterized to investigate the role of photorespiration in C4 photosynthesis. An Activator-induced allele of GO1 conditioned a seedling lethal phenotype when homozygous and had 5% to 10% of wild-type GO activity. Growth of seedlings in high CO2 (1%-5%) was sufficient to rescue the mutant phenotype. Upon transfer to normal air,...
متن کاملDirect expression of active spinach glycolate oxidase in Escherichia coli.
Spinach glycolate oxidase (GAO) was expressed in Escherichia coli using the T7 RNA polymerase promotor. The enzyme accounts for approx. 1% of the soluble protein fraction and is expressed as a soluble and active enzyme. Comparison with GAO expressed in Saccharomyces cerevisiae (Macheroux, P., Massey, V., Thiele, D.J. and Volokita, M. (1991) Biochemistry 30, 4612-4619) showed that the GAO expres...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1968
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.43.2.289